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p63 isoforms pentr twist backbone  (Twist Bioscience)

 
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    Twist Bioscience p63 isoforms pentr twist backbone
    A) Schematic of TAp63 and ΔNp63 C-terminal TAD mutants. B) Protein expression of C-terminal TAD mutants in pcDNA plasmid constructs transiently transfected in HCT116 TP53 -/- cells. Negative control is an empty pcDNA backbone. C) Reporter assay of TAp63 and D) ΔNp63 C-terminal TAD mutants on a p63 RE (green) and a mutant (grey) p63 RE. E) Protein expression of ΔNp63 C-terminal TAD mutants in lentiviral vectors under 24hr doxycycline induction in HCT116 TP53 -/- cells. Negative control is GUS expressed in <t>pCW57.1</t> vector. F) QRT-PCR analysis of KRT5 expression by ΔNp63 C-terminal TAD mutants G) QRT-PCR analysis of JAG2 expression by ΔNp63 C-terminal TAD mutants. H) Genome browser view of KRT5 locus displaying p63 and H3K27ac ChIP-seq binding data. I) Genome browser view of JAG2 locus displaying p63 and H3K27ac ChIP-seq binding data. Statistical analysis for qRT-PCR data was done using a a One-way ANOVA test (*: p -value <.05, **: p -value <.01, ***: p -value <.001, ****: p -value < 0.0001, ns = not significant) and a Two-way ANOVA ( ***: p -value <.001, ****: p -value < 0.0001, ns = not significant ) for reporter assay data.
    P63 Isoforms Pentr Twist Backbone, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p63+isoforms+pentr+twist+backbone/p63+isoforms+pentr+twist+backbone/bio_rxiv__2024__12__03__626646-235-1-8
    Average 90 stars, based on 1 article reviews
    p63 isoforms pentr twist backbone - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Differential transcriptional activity of ΔNp63β is encoded by an isoform-specific C-terminus"

    Article Title: Differential transcriptional activity of ΔNp63β is encoded by an isoform-specific C-terminus

    Journal: bioRxiv

    doi: 10.1101/2024.12.03.626646

    A) Schematic of TAp63 and ΔNp63 C-terminal TAD mutants. B) Protein expression of C-terminal TAD mutants in pcDNA plasmid constructs transiently transfected in HCT116 TP53 -/- cells. Negative control is an empty pcDNA backbone. C) Reporter assay of TAp63 and D) ΔNp63 C-terminal TAD mutants on a p63 RE (green) and a mutant (grey) p63 RE. E) Protein expression of ΔNp63 C-terminal TAD mutants in lentiviral vectors under 24hr doxycycline induction in HCT116 TP53 -/- cells. Negative control is GUS expressed in pCW57.1 vector. F) QRT-PCR analysis of KRT5 expression by ΔNp63 C-terminal TAD mutants G) QRT-PCR analysis of JAG2 expression by ΔNp63 C-terminal TAD mutants. H) Genome browser view of KRT5 locus displaying p63 and H3K27ac ChIP-seq binding data. I) Genome browser view of JAG2 locus displaying p63 and H3K27ac ChIP-seq binding data. Statistical analysis for qRT-PCR data was done using a a One-way ANOVA test (*: p -value <.05, **: p -value <.01, ***: p -value <.001, ****: p -value < 0.0001, ns = not significant) and a Two-way ANOVA ( ***: p -value <.001, ****: p -value < 0.0001, ns = not significant ) for reporter assay data.
    Figure Legend Snippet: A) Schematic of TAp63 and ΔNp63 C-terminal TAD mutants. B) Protein expression of C-terminal TAD mutants in pcDNA plasmid constructs transiently transfected in HCT116 TP53 -/- cells. Negative control is an empty pcDNA backbone. C) Reporter assay of TAp63 and D) ΔNp63 C-terminal TAD mutants on a p63 RE (green) and a mutant (grey) p63 RE. E) Protein expression of ΔNp63 C-terminal TAD mutants in lentiviral vectors under 24hr doxycycline induction in HCT116 TP53 -/- cells. Negative control is GUS expressed in pCW57.1 vector. F) QRT-PCR analysis of KRT5 expression by ΔNp63 C-terminal TAD mutants G) QRT-PCR analysis of JAG2 expression by ΔNp63 C-terminal TAD mutants. H) Genome browser view of KRT5 locus displaying p63 and H3K27ac ChIP-seq binding data. I) Genome browser view of JAG2 locus displaying p63 and H3K27ac ChIP-seq binding data. Statistical analysis for qRT-PCR data was done using a a One-way ANOVA test (*: p -value <.05, **: p -value <.01, ***: p -value <.001, ****: p -value < 0.0001, ns = not significant) and a Two-way ANOVA ( ***: p -value <.001, ****: p -value < 0.0001, ns = not significant ) for reporter assay data.

    Techniques Used: Expressing, Plasmid Preparation, Construct, Transfection, Negative Control, Reporter Assay, Mutagenesis, Quantitative RT-PCR, ChIP-sequencing, Binding Assay

    Related Articles

    Clone Assay:

    Article Title: Differential transcriptional activity of ΔNp63β is encoded by an isoform-specific C-terminus
    Article Snippet: .. For pCW57.1, p63 isoforms in pENTR Twist backbone (Twist Biosciences) were cloned via Gateway cloning using LR Clonase enzyme. .. All mutants were cloned via site directed-mutagenesis or HiFi assembly and full plasmid sequencing was performed using Plasmidsaurus.

    Cloning:

    Article Title: Differential transcriptional activity of ΔNp63β is encoded by an isoform-specific C-terminus
    Article Snippet: .. For pCW57.1, p63 isoforms in pENTR Twist backbone (Twist Biosciences) were cloned via Gateway cloning using LR Clonase enzyme. .. All mutants were cloned via site directed-mutagenesis or HiFi assembly and full plasmid sequencing was performed using Plasmidsaurus.



    Similar Products

    p63 isoforms pentr twist backbone  (Twist Bioscience)
    90
    Twist Bioscience p63 isoforms pentr twist backbone
    A) Schematic of TAp63 and ΔNp63 C-terminal TAD mutants. B) Protein expression of C-terminal TAD mutants in pcDNA plasmid constructs transiently transfected in HCT116 TP53 -/- cells. Negative control is an empty pcDNA backbone. C) Reporter assay of TAp63 and D) ΔNp63 C-terminal TAD mutants on a p63 RE (green) and a mutant (grey) p63 RE. E) Protein expression of ΔNp63 C-terminal TAD mutants in lentiviral vectors under 24hr doxycycline induction in HCT116 TP53 -/- cells. Negative control is GUS expressed in <t>pCW57.1</t> vector. F) QRT-PCR analysis of KRT5 expression by ΔNp63 C-terminal TAD mutants G) QRT-PCR analysis of JAG2 expression by ΔNp63 C-terminal TAD mutants. H) Genome browser view of KRT5 locus displaying p63 and H3K27ac ChIP-seq binding data. I) Genome browser view of JAG2 locus displaying p63 and H3K27ac ChIP-seq binding data. Statistical analysis for qRT-PCR data was done using a a One-way ANOVA test (*: p -value <.05, **: p -value <.01, ***: p -value <.001, ****: p -value < 0.0001, ns = not significant) and a Two-way ANOVA ( ***: p -value <.001, ****: p -value < 0.0001, ns = not significant ) for reporter assay data.
    P63 Isoforms Pentr Twist Backbone, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p63+isoforms+pentr+twist+backbone/p63+isoforms+pentr+twist+backbone/bio_rxiv__2024__12__03__626646-235-1-8
    Average 90 stars, based on 1 article reviews
    p63 isoforms pentr twist backbone - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Schematic of TAp63 and ΔNp63 C-terminal TAD mutants. B) Protein expression of C-terminal TAD mutants in pcDNA plasmid constructs transiently transfected in HCT116 TP53 -/- cells. Negative control is an empty pcDNA backbone. C) Reporter assay of TAp63 and D) ΔNp63 C-terminal TAD mutants on a p63 RE (green) and a mutant (grey) p63 RE. E) Protein expression of ΔNp63 C-terminal TAD mutants in lentiviral vectors under 24hr doxycycline induction in HCT116 TP53 -/- cells. Negative control is GUS expressed in pCW57.1 vector. F) QRT-PCR analysis of KRT5 expression by ΔNp63 C-terminal TAD mutants G) QRT-PCR analysis of JAG2 expression by ΔNp63 C-terminal TAD mutants. H) Genome browser view of KRT5 locus displaying p63 and H3K27ac ChIP-seq binding data. I) Genome browser view of JAG2 locus displaying p63 and H3K27ac ChIP-seq binding data. Statistical analysis for qRT-PCR data was done using a a One-way ANOVA test (*: p -value <.05, **: p -value <.01, ***: p -value <.001, ****: p -value < 0.0001, ns = not significant) and a Two-way ANOVA ( ***: p -value <.001, ****: p -value < 0.0001, ns = not significant ) for reporter assay data.

    Journal: bioRxiv

    Article Title: Differential transcriptional activity of ΔNp63β is encoded by an isoform-specific C-terminus

    doi: 10.1101/2024.12.03.626646

    Figure Lengend Snippet: A) Schematic of TAp63 and ΔNp63 C-terminal TAD mutants. B) Protein expression of C-terminal TAD mutants in pcDNA plasmid constructs transiently transfected in HCT116 TP53 -/- cells. Negative control is an empty pcDNA backbone. C) Reporter assay of TAp63 and D) ΔNp63 C-terminal TAD mutants on a p63 RE (green) and a mutant (grey) p63 RE. E) Protein expression of ΔNp63 C-terminal TAD mutants in lentiviral vectors under 24hr doxycycline induction in HCT116 TP53 -/- cells. Negative control is GUS expressed in pCW57.1 vector. F) QRT-PCR analysis of KRT5 expression by ΔNp63 C-terminal TAD mutants G) QRT-PCR analysis of JAG2 expression by ΔNp63 C-terminal TAD mutants. H) Genome browser view of KRT5 locus displaying p63 and H3K27ac ChIP-seq binding data. I) Genome browser view of JAG2 locus displaying p63 and H3K27ac ChIP-seq binding data. Statistical analysis for qRT-PCR data was done using a a One-way ANOVA test (*: p -value <.05, **: p -value <.01, ***: p -value <.001, ****: p -value < 0.0001, ns = not significant) and a Two-way ANOVA ( ***: p -value <.001, ****: p -value < 0.0001, ns = not significant ) for reporter assay data.

    Article Snippet: For pCW57.1, p63 isoforms in pENTR Twist backbone (Twist Biosciences) were cloned via Gateway cloning using LR Clonase enzyme.

    Techniques: Expressing, Plasmid Preparation, Construct, Transfection, Negative Control, Reporter Assay, Mutagenesis, Quantitative RT-PCR, ChIP-sequencing, Binding Assay